Go to http://cbl-gorilla.cs.technion.ac.il/
Paste your gene list separated by /n
Chose the species
Chose running mode: Single ranked list of genes
In case you got as an output a ranked list of genes paste in total list of your genes in a ranked order (e.g. in you had 20.000 genes tested and ranked in your experiment, paste in all 20.000 genes in the order of ranking).
Chose an ontology: All
Select all 3 groups of ontologies to be shown as output.
The output is a table:
|null||Description||P-value||Enrichment (N, B, n, b)||Genes|
|GO:0030695||GTPase regulator activity||5.45E-6||1.72 (13439,402,1552,80)||[+] Show genes|
|GO:0060589||nucleoside-triphosphatase regulator activity||7.46E-6||1.70 (13439,412,1552,81)||[+] Show genes|
|GO:0005488||binding||3.36E-5||1.07 (13439,9544,1560,1185)||[+] Show genes|
|GO:0042578||phosphoric ester hydrolase activity||2.42E-4||1.83 (13439,311,1110,47)||[+] Show genes|
|GO:0005509||calcium ion binding||3.77E-4||1.49 (13439,515,1591,91)||[+] Show genes|
|GO:0005083||small GTPase regulator activity||4.58E-4||1.71 (13439,258,1552,51)||[+] Show genes|
|GO:0005096||GTPase activator activity||4.66E-4||1.75 (13439,233,1552,47)||[+] Show genes|
|GO:0005085||guanyl-nucleotide exchange factor activity||6.01E-4||1.91 (13439,161,1484,34)||[+] Show genes|
|GO:0047555||3',5'-cyclic-GMP phosphodiesterase activity||6.25E-4||11.71 (13439,7,656,4)||[+] Show genes|
|GO:0005515||protein binding||8.63E-4||1.12 (13439,5545,1458,672)||[+] Show genes|
In case you don't have a ranked list of genes, but just a group of genes that are came out of the experiment, select as running mode: Two unranked lists of genes (target and background lists).
In this case you will need to provide a background list of genes for the analysis. If for example your gene list comes from a microarray experiment the background should be all the genes from the array.